GENERALIZED TRANSDUCTION IN CAULOBACTER CRESCENTUS
Author(s) -
Bert Ely,
Reid C. Johnson
Publication year - 1977
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/87.3.391
Subject(s) - caulobacter crescentus , transduction (biophysics) , lysogenic cycle , bacteriophage , biology , virulence , genetics , microbiology and biotechnology , bacteria , bacterial protein , gene , escherichia coli , biochemistry
Two closely related bacteriophage, φCr30 and φCr35, are the first bacteriophage shown to mediate generalized transduction in Caulobacter crescentus. Unlike most other transducing phage, they are virulent and do not form any sort of lysogenic relationship with their host. However, they are rather inefficient at adsorption, so that transductants have a good chance of survival. The phage particles have a head 80 nm in diameter and a contractile tail 140 nm in length. Procedures for growth and transduction with φCr30 are relatively simple; thus, it will be of great value for the genetic analysis of C. crescentus.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom