ISOLATION OF SPONTANEOUSLY DERIVED MUTANTS OF CAULOBACTER CRESCENTUS | Zendy

Reid C. Johnson | Zendy; Bert Ely | Zendy

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ISOLATION OF SPONTANEOUSLY DERIVED MUTANTS OF CAULOBACTER CRESCENTUS

Author(s) -

Reid C. Johnson,

Bert Ely

Publication year - 1977

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/86.1.25

Subject(s) - caulobacter crescentus , biology , isolation (microbiology) , genetics , mutant , mutation , strain (injury) , phenotype , microbiology and biotechnology , bacterial protein , bacteria , gene , anatomy

Caulobacter crescentus has a penicillinase which precludes the use of penicillin for mutant enrichment. However, two other antibiotics, fosfomycin and D-cycloserine, can be used to enrich for C. crescentus mutants. In enrichment procedures for C. crescentus auxotrophs, spontaneously derived mutants occur at a frequency of 5–10% among the survivors of an enrichment procedure. Consequently, large numbers of mutants are readily obtained without any need for mutagenesis. These mutants are heterogeneous both with regard to the type of mutation and to the nutritional requirement. A similar procedure has been used to isolate temperature-sensitive mutants.

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