ALCOHOL DEHYDROGENASE-NEGATIVE MUTANTS IN DROSOPHILA: DEFECTS AT THE STRUCTURAL LOCUS? | Zendy

Marcia Schwartz | Zendy; William Sofer | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

ALCOHOL DEHYDROGENASE-NEGATIVE MUTANTS IN DROSOPHILA: DEFECTS AT THE STRUCTURAL LOCUS?

Author(s) -

Marcia Schwartz,

William Sofer

Publication year - 1976

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/83.1.125

Subject(s) - alcohol dehydrogenase , complementation , biology , mutant , structural gene , ethyl methanesulfonate , alcohol oxidoreductase , locus (genetics) , genetics , gene , enzyme , sodium dodecyl sulfate , biochemistry , gel electrophoresis , microbiology and biotechnology , nad+ kinase

Sixteen Adh-negative mutants (induced by ethyl methanesulfonate) were examined for the presence of inactive alcohol dehydrogenase (ADH) protein. Four techniques were utilized in an effort to detect this protein: hybrid enzyme formation, intra-cistronic complementation, sodium dodecyl sulfate electrophoresis and antibody precipitation. Eleven of the sixteen negative strains showed evidence of inactive ADH protein and are presumably mutations in the structural element. These results are discussed in light of some recent models of gene organization in higher organisms.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research