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An enrichment procedure has been developed that results in at least a 200× increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N′-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.

MUTANT ENRICHMENT IN THE COLONIAL ALGA, EUDORINA ELEGANS