MUTANT ENRICHMENT IN THE COLONIAL ALGA, EUDORINA ELEGANS | Zendy

Anne L. Toby | Zendy; C. Lindley Kemp | Zendy

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MUTANT ENRICHMENT IN THE COLONIAL ALGA, EUDORINA ELEGANS

Author(s) -

Anne L. Toby,

C. Lindley Kemp

Publication year - 1975

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/81.2.243

Subject(s) - mutant , biology , mutation , genetics , wild type , starvation , nitrogen , nitro , biochemistry , microbiology and biotechnology , gene , chemistry , endocrinology , alkyl , organic chemistry

An enrichment procedure has been developed that results in at least a 200× increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N′-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.

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