REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA. I. HOT SPOT ACTIVITY ASSOCIATED WITH SPI-DELETIONS AND bio SUBSTITUTIONS | Zendy

Kenneth D. McMilin | Zendy; Mary M. Stahl | Zendy; Franklin W. Stahl | Zendy

Open Access

REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA. I. HOT SPOT ACTIVITY ASSOCIATED WITH SPI-DELETIONS AND bio SUBSTITUTIONS

Author(s) -

Kenneth D. McMilin,

Mary M. Stahl,

Franklin W. Stahl

Publication year - 1974

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/77.3.409

Subject(s) - recombination , biology , genetics , bacteriophage , gene , genetic recombination , chromosome , hot spot (computer programming) , lambda phage , recombinant dna , dna , microbiology and biotechnology , escherichia coli , computer science , operating system

In order to survey the distribution along the bacteriophage λ chromosome of Rec-mediated recombination events, crosses are performed using conditions which block essentially all DNA synthesis. One parent is density-labeled and carries a genetic marker in the left terminal λ gene (A), while the other parent is unlabeled and carries a genetic marker in the right terminal λ gene (R). Both parents are deleted for the λ recombination genes int and red, together with other recombination-associated genes, by virtue of either (1) a pure deletion or (2) a bio insertion-deletion. The distribution in a cesium density gradient of the resulting A+R+ recombinant phage reflects the chromosomal distribution of the recombination events which gave rise to those phage.

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