Open AccessGENETIC CONTROL OF CHROMOSOMAL AND PLASMID RECOMBINATION IN STAPHYLOCOCCUS AUREUS
Author(s) -
Lorraine Wyman,
Richard V. Goering,
Richard P. Novick
Publication year - 1974
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/76.4.681
Subject(s) - recombination , biology , genetic recombination , plasmid , genetics , flp frt recombination , bacteriophage , mutant , site specific recombination , escherichia coli , cre lox recombination , staphylococcus aureus , lysogenic cycle , dna , microbiology and biotechnology , gene , bacteria , transgene , recombinase , genetically modified mouse
Recombination-deficient mutants of Staphylococcus aureus have been isolated and found to have properties similar to those of recombination-deficient Escherichia coli. In addition, one Rec- mutant was found to be defective in the restriction and modification of DNA. There is a marked reduction (∼ 104-fold) in recombination between penicillinase plasmids in the Rec- mutants suggesting that these elements do not encode an efficient recombination system. There is, however, a demonstrable residuum of interplasmid recombination; evidence is lacking on whether this residuum is a plasmid or host function. In the absence of the generalized host recombination system it has been possible to demonstrate that interplasmid recombination occurs during vegetative bacteriophage growth and is presumably mediated by a phage-determined recombination system.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom