ISOLATION OF P22 SPECIALIZED TRANSDUCING PHAGE FOLLOWING F'-EPISOME FUSION | Zendy

Robert Kaye | Zendy; John Barravecchio | Zendy; John R. Roth | Zendy

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ISOLATION OF P22 SPECIALIZED TRANSDUCING PHAGE FOLLOWING F'-EPISOME FUSION

Author(s) -

Robert Kaye,

John Barravecchio,

John R. Roth

Publication year - 1974

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/76.4.655

Subject(s) - lysogen , prophage , biology , genetics , bacteriophage , lysogenic cycle , ornithine transcarbamylase , gene , ornithine carbamoyltransferase , escherichia coli , ornithine , amino acid , arginine , urea cycle

A P22 specialized transducing phage has been constructed which carries the structural gene for aspartate transcarbamylase (ATCase). This gene (pyrB) was first brought close to the P22 attachment site by fusing an F' pyrB + episome to an F' prolac episome which carries a P22 prophage attachment site. A prophage was added to these fused F' episomes and the lysogen was UV-induced. The specialized transducing phage was isolated from the resulting lysate. The phage also carries argI, the structure gene for ornithine transcarbamylase.

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