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Methods for genetically mapping the end points of tandem duplicatictions of the rII region of bacteriophage T4D are presented. Analysis of ten duplications indicates (1) that the position of duplication end points and therefore the length of the duplicated segment differ in strains of independent origin; (2) that there is a direct relationship between segregation frequency and length; (3) that segregation is more frequent than expected on the basis of standard genetic mapping; and (4) that while duplications frequently include non-rII genetic material, frequently they do not include the entire rII region. The duplications studied range from less than two to about five cistrons in length.

THE GENETIC CONSTITUTION OF TANDEM DUPLICATIONS OF THE rII OF BACTERIOPHAGE T4D