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With the phosphatase inhibitor, okadaic acid, we induce the precocious onset of the chiasmate stage and under those conditions show that the recombination nodules, MLH1 and MLH3 foci, are localized to the chiasmata. It is concluded that MLH1/3 foci are appropriate markers for the studies of crossovers/chiasmata development and distribution at late meiotic prophase.

MLH1p and MLH3p Localize to Precociously Induced Chiasmata of Okadaic-Acid-Treated Mouse Spermatocytes