The Drosophila melanogaster importin α3 Locus Encodes an Essential Gene Required for the Development of Both Larval and Adult Tissues

The nuclear transport of classical nuclear localization signal (cNLS)-containing proteins is mediated by the cNLS receptor importin α. The conventional importin α gene family in metazoan animals is composed of three clades that are conserved between flies and mammals and are referred to here as α1, α2, and α3. In contrast, plants and fungi contain only α1 genes. In this study we report that Drosophila importin α3 is required for the development of both larval and adult tissues. Importin α3 mutant flies die around the transition from first to second instar larvae, and homozygous importin α3 mutant eyes are defective. The transition to second instar larvae was rescued with importin α1, α2, or α3 transgenes, indicating that Importin α3 is normally required at this stage for an activity shared by all three importin α's. In contrast, an α3-specific biochemical activity(s) of Importin α3 is probably required for development to adults and photoreceptor cell development, since only an importin α3 transgene rescued these processes. These results are consistent with the view that the importin α's have both overlapping and distinct functions and that their role in animal development involves the spatial and temporal control of their expression.