Genetic and Physical Mapping of Avr1a in Phytophthora sojae | Zendy

Terry MacGregor | Zendy; Madan K. Bhattacharyya | Zendy; Brett M. Tyler | Zendy; Ravindra G. Bhat | Zendy; A. F. Schmitthenner | Zendy; Mark Gijzen | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Genetic and Physical Mapping of Avr1a in Phytophthora sojae

Author(s) -

Terry MacGregor,

Madan K. Bhattacharyya,

Brett M. Tyler,

Ravindra G. Bhat,

A. F. Schmitthenner,

Mark Gijzen

Publication year - 2002

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/160.3.949

Subject(s) - phytophthora sojae , biology , contig , genetics , genetic marker , locus (genetics) , rapd , population , gene mapping , genetic linkage , amplified fragment length polymorphism , gene , oomycete , phytophthora , quantitative trait locus , genotype , genome , botany , genetic diversity , chromosome , demography , sociology

The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F2 populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F2 populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F2 population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F2 progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research