Isolation of X and Y Chromosome-Specific DNA Markers From a Liverwort, Marchantia polymorpha, by Representational Difference Analysis
Author(s) -
Masaki Fujisawa,
Kiwako Hayashi,
Tomohisa Nishio,
Tomoyuki Bando,
Sachiko Okada,
Katsuyuki T. Yamato,
Hideya Fukuzawa,
Kanji Ohyama
Publication year - 2001
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/159.3.981
Subject(s) - marchantia polymorpha , biology , genetics , dna , chromosome , y chromosome , isolation (microbiology) , gene , bioinformatics
The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.
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