A Simple Sequence Repeat-Based Linkage Map of Barley | Zendy

Luke Ramsay | Zendy; Malcolm Macaulay | Zendy; Stephania degli Ivanissevich | Zendy; Kenneth N. Maclean | Zendy; Linda Cardle | Zendy; John Fuller | Zendy; Keith J. Edwards | Zendy; Stig Tuvesson | Zendy; Michele Morgante | Zendy; A. Massari | Zendy; Elena Maestri | Zendy; Nelson Marmiroli | Zendy; Tatjana Sjakste | Zendy; Martin W. Ganal | Zendy; W. Powell | Zendy; Robbie Waugh | Zendy

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A Simple Sequence Repeat-Based Linkage Map of Barley

Author(s) -

Luke Ramsay,

Malcolm Macaulay,

Stephania degli Ivanissevich,

Kenneth N. Maclean,

Linda Cardle,

John Fuller,

Keith J. Edwards,

Stig Tuvesson,

Michele Morgante,

A. Massari,

Elena Maestri,

Nelson Marmiroli,

Tatjana Sjakste,

Martin W. Ganal,

W. Powell,

Robbie Waugh

Publication year - 2000

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/156.4.1997

Subject(s) - biology , genetics , microsatellite , hordeum vulgare , population , gene mapping , genetic linkage , ploidy , genetic marker , primer (cosmetics) , restriction fragment length polymorphism , chromosome , polymerase chain reaction , gene , botany , poaceae , allele , chemistry , demography , organic chemistry , sociology

A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F1 of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci. Centromeric clustering of markers was observed in the main mapping population; however, the clustering severity was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.

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