The Mla (Powdery Mildew) Resistance Cluster Is Associated With Three NBS-LRR Gene Families and Suppressed Recombination Within a 240-kb DNA Interval on Chromosome 5S (1HS) of Barley | Zendy

Fusheng Wei | Zendy; Karin Sue Gobelman-Werner | Zendy; Shaun Morroll | Zendy; Joachim Kurth | Zendy; Long Mao | Zendy; Rod A. Wing | Zendy; Dario Leister | Zendy; Paul SchulzeLefert | Zendy; Roger P. Wise | Zendy

Open Access

The Mla (Powdery Mildew) Resistance Cluster Is Associated With Three NBS-LRR Gene Families and Suppressed Recombination Within a 240-kb DNA Interval on Chromosome 5S (1HS) of Barley

Author(s) -

Fusheng Wei,

Karin Sue Gobelman-Werner,

Shaun Morroll,

Joachim Kurth,

Long Mao,

Rod A. Wing,

Dario Leister,

Paul SchulzeLefert,

Roger P. Wise

Publication year - 1999

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/153.4.1929

Subject(s) - biology , powdery mildew , genetics , contig , gene , gene cluster , chromosome , gene mapping , genome , population , botany , demography , sociology

Powdery mildew of barley, caused by Erysiphe graminis f. sp. hordei, is a model system for investigating the mechanism of gene-for-gene interaction between large-genome cereals and obligate-fungal pathogens. A large number of loci that confer resistance to this disease are located on the short arm of chromosome 5(1H). The Mla resistance-gene cluster is positioned near the telomeric end of this chromosome arm. AFLP-, RAPD-, and RFLP-derived markers were used to saturate the Mla region in a high-resolution recombinant population segregating for the (Mla6 + Mla14) and (Mla13 + Ml-Ru3) resistance specificities. These tightly linked genetic markers were used to identify and develop a physical contig of YAC and BAC clones spanning the Mla cluster. Three distinct NBS-LRR resistance-gene homologue (RGH) families were revealed via computational analysis of low-pass and BAC-end sequence data derived from Mla-spanning clones. Genetic and physical mapping delimited the Mla-associated, NBS-LRR gene families to a 240-kb interval. Recombination within the RGH families was at least 10-fold less frequent than between markers directly adjacent to the Mla cluster.

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