A Pseudoautosomal Random Amplified Polymorphic DNA Marker for the Sex Chromosomes of Silene dioica | Zendy

Verónica S. Di Stilio | Zendy; Richard V. Kesseli | Zendy; David L. Mulcahy | Zendy

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A Pseudoautosomal Random Amplified Polymorphic DNA Marker for the Sex Chromosomes of Silene dioica

Author(s) -

Verónica S. Di Stilio,

Richard V. Kesseli,

David L. Mulcahy

Publication year - 1998

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/149.4.2057

Subject(s) - pseudoautosomal region , biology , genetics , nondisjunction , bulked segregant analysis , rapd , x chromosome , y chromosome , genetic marker , w chromosome , chromosome , gene , gene mapping , population , aneuploidy , karyotype , demography , sociology , genetic diversity

The segregation pattern of an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Silene dioica (L.) Clairv. family provides evidence that this molecular marker is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The marker was found through a combination of bulked segregant analysis (BSA) and RAPD techniques. Recombination rates between this pseudoautosomal marker and the differentiating portion of the Y chromosome are 15% in both generations. Alternative explanations involving nondisjunction or autosomal inheritance are presented and discussed. Chromosome counts provide evidence against the nondisjunction hypothesis, and probability calculations argue against the possibility of autosomal inheritance. This constitutes the first report of a pseudoautosomal DNA marker for plant sex chromosomes.

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