A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus) | Zendy

Thomas D. Kocher | Zendy; Woo-Jai Lee | Zendy; Halina Sobolewska | Zendy; David J. Penman | Zendy; Brendan J. McAndrew | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis niloticus)

Author(s) -

Thomas D. Kocher,

Woo-Jai Lee,

Halina Sobolewska,

David J. Penman,

Brendan J. McAndrew

Publication year - 1998

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/148.3.1225

Subject(s) - biology , microsatellite , cichlid , oreochromis , genetics , genetic linkage , tilapia , ploidy , evolutionary biology , genetic marker , fish <actinopterygii> , gene , fishery , allele

We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of ~1000–1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research