DNA synthesis errors associated with double-strand-break repair. | Zendy

Jeffrey N. Strathern | Zendy; B K Shafer | Zendy; Carolyn McGill | Zendy

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DNA synthesis errors associated with double-strand-break repair.

Author(s) -

Jeffrey N. Strathern,

B K Shafer,

Carolyn McGill

Publication year - 1995

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/140.3.965

Subject(s) - biology , reversion , dna , microbiology and biotechnology , gene , genetics , gene duplication , dna repair , allele , saccharomyces cerevisiae , endonuclease , phenotype

Repair of a site-specific double-strand DNA break (DSB) resulted in increased reversion frequency for a nearby allele. Site-specific DSBs were introduced into the genome of Saccharomyces cerevisiae by the endonuclease encoded by the HO gene. Expression of the HO gene from a galactose-inducible promoter allowed efficient DNA cleavage at a single site in large populations of cells. To determine whether the DNA synthesis associated with repair of DSBs has a higher error rate than that associated with genome duplication, HO-induced DSBs were generated 0.3 kb from revertible alleles of trp1. The reversion rate of the trp1 alleles was approximately 100-fold higher among cells that had experienced an HO cut than among uninduced cells. The reverted allele was found predominantly on the chromosome that experienced the DNA cleavage.

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