Open AccessA genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS).
Author(s) -
Yoshihide Hayashizaki,
Shinji Hirotsune,
Yasushi Okazaki,
Hideshi Shibata,
A Akasako,
Masami Muramatsu,
Jun Kawai,
Tsutomu Hirasawa,
Sachihiko Watanabe,
T Shiroishi
Publication year - 1994
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/138.4.1207
Subject(s) - biology , genetics , genome , restriction enzyme , gene mapping , genetic linkage , restriction fragment , restriction fragment length polymorphism , restriction map , quantitative trait locus , genomic dna , chromosome , gene , polymerase chain reaction , plasmid
We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled restriction fragments of genomic DNA that are separated by using high resolution, two-dimensional gel electrophoresis identifying as many as two thousand landmark loci in a single gel. Variation for several hundred of these loci has been identified between laboratory strains and between these strains and Mus spretus. The segregation of more than 1100 RLGS loci has been analyzed in recombinant inbred (RI) strains and in two separate interspecific genetic crosses. Genetic maps have been derived that link 1045 RLGS loci to reference loci on all of the autosomes and the X chromosome of the mouse genome. The RLGS method can be applied to genome analysis in many different organisms to identify genomic loci because it uses end-labeling of restriction landmarks rather than probe hybridization. Different combinations of restriction enzymes yield different sets of RLGS loci providing expanded power for genetic mapping.