Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. | Zendy

Rick Kesseli | Zendy; Ilan Paran | Zendy; Richard W. Michelmore | Zendy

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Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers.

Author(s) -

Rick Kesseli,

Ilan Paran,

Richard W. Michelmore

Publication year - 1994

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/136.4.1435

Subject(s) - rapd , biology , genetics , restriction fragment length polymorphism , lactuca , genetic marker , genetic linkage , gene mapping , genome , population , genetic diversity , gene , botany , chromosome , polymerase chain reaction , demography , sociology

A detailed genetic map has been constructed from the F2 population of a single intraspecific cross of Lactuca sativa (n = 9). It comprises 319 loci, including 152 restriction fragment length polymorphism (RFLP), 130 random amplified polymorphic DNA (RAPD), 7 isozyme, 19 disease resistance, and 11 morphological markers. Thirteen major, four minor linkage groups and several unlinked markers are identified for this genome which is estimated to be approximately 1950 cM. RFLP and RAPD markers show similar distributions throughout the genome and identified similar levels of polymorphism. RAPD loci were much quicker to identify but more difficult to order. Procedures for generating accurate genetic maps and their limitations are described.

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