Chromosome rearrangements recovered following transformation of Neurospora crassa. | Zendy

Dmitri Perkins | Zendy; John Á. Kinsey | Zendy; David K. Asch | Zendy; Gregory D. Frederick | Zendy

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Chromosome rearrangements recovered following transformation of Neurospora crassa.

Author(s) -

Dmitri Perkins,

John Á. Kinsey,

David K. Asch,

Gregory D. Frederick

Publication year - 1993

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/134.3.729

Subject(s) - biology , neurospora crassa , genetics , breakpoint , chromosomal translocation , locus (genetics) , chromosomal rearrangement , chromosome , southern blot , transformation (genetics) , crassa , homologous chromosome , gene rearrangement , microbiology and biotechnology , gene , karyotype , mutant

New chromosome rearrangements were found in 10% or more of mitotically stable transformants. This was shown for transformations involving a variety of different markers, vectors and recipient strains. Breakpoints were randomly distributed among the seven linkage groups. Controls using untransformed protoplasts of the same strains contained almost no rearrangements. A study of molecularly characterized Am+ transformants showed that rearrangements are frequent when multiple ectopic integration events have occurred. In contrast, rearrangements are absent or infrequent when only the resident locus is restored to am+ by a homologous event. Sequences of the transforming vector were genetically linked to breakpoints in 6 of 10 translocations that were examined using Southern hybridization or colony blots.

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