Genetic recombination in bacteriophage T4: single-burst analysis of cosegregants and evidence in favor of a splice/patch coupling model. | Zendy

Victor P. Shcherbakov | Zendy; L A Plugina | Zendy; M A Nesheva | Zendy

Open Access

Genetic recombination in bacteriophage T4: single-burst analysis of cosegregants and evidence in favor of a splice/patch coupling model.

Author(s) -

Victor P. Shcherbakov,

L A Plugina,

M A Nesheva

Publication year - 1992

Publication title -

genetics.

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

ISSN - 3049-7094

DOI - 10.1093/genetics/131.4.769

Subject(s) - recombination , biology , genetics , genetic recombination , homologous recombination , splice , recombinant dna , bacteriophage , dna , chromosome , coupling (piping) , chromosomal crossover , microbiology and biotechnology , gene , escherichia coli , mechanical engineering , engineering

To reveal the structure of penultimate DNA intermediates in T4 bacteriophage recombination, resolution of which produces free recombinant molecules, a single-burst analysis of the recombinant progeny was made in multifactor crosses, enabling one to determine quantitatively the different recombinants generated by one or two exchanges within the same chromosome segment. It was found that double and single exchanges are highly correlated in T4 recombination. These results were interpreted as evidence for simultaneous formation of a splice/patch pair as the primary recombination products. A recombination model called here the "splice/patch coupling model" is presented according to which resolution of a single DNA intermediate results in two linear heterozygous molecules containing a patch and a splice, respectively, in homologous positions.

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