Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome. | Zendy

Brian I. Osborne | Zendy; Catherine Corr | Zendy; James P. Prince | Zendy; Reinhard Hehl | Zendy; Steven D. Tanksley | Zendy; Susan P. McCormick | Zendy; Barbara Baker | Zendy

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Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome.

Author(s) -

Brian I. Osborne,

Catherine Corr,

James P. Prince,

Reinhard Hehl,

Steven D. Tanksley,

Susan P. McCormick,

Barbara Baker

Publication year - 1991

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/129.3.833

Subject(s) - biology , genetics , transposition (logic) , genome , restriction fragment length polymorphism , dna , gene , restriction map , restriction fragment , transposable element , nucleic acid sequence , microbiology and biotechnology , genotype , philosophy , linguistics

We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds.

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