Recombination in the B chromosome of maize to produce A-B-A chromosomes. | Zendy

James A. Birchler | Zendy; D J Chalfoun | Zendy; D M Levin | Zendy

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Recombination in the B chromosome of maize to produce A-B-A chromosomes.

Author(s) -

James A. Birchler,

D J Chalfoun,

D M Levin

Publication year - 1990

Publication title -

genetics.

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

ISSN - 3049-7094

DOI - 10.1093/genetics/126.3.723

Subject(s) - biology , chromosomal translocation , genetics , b chromosome , meiosis , aleurone , centromere , chromosome , small supernumerary marker chromosome , chromosomal crossover , chiasma , karyotype , endosperm , gene , chromosome 22

The translocations between the supernumerary B chromosomes and the normal A chromosomes of maize provide a valuable tool for gene localizations, dosage studies and characterization of mutants as null, leaky or gain-of-function. A procedure is described, that relies on recombination in the B chromosome, for marking each of the various B-A translocations with a single dominant marker that will allow dosage classifications of individuals at the mature kernel stage. This marker is R-scm3, which conditions anthocyanin pigment in the aleurone of the endosperm and the scutellum of the embryo. A test for recombination in the B chromosome was conducted by crossing together two translocations, that were broken on opposite sides of the B centromere, and in different A chromosome arms, namely TB-1La and TB-10L18. An example was recovered that linked genetic markers on 1L and 10L to the B centromere. Cytological examination at pachytene of meiosis confirmed the new chromosomal linkage. The use of this procedure to produce a comprehensive set of uniformly marked B-A translocations is discussed.

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