Construction of an Unstable Ring-X Chromosome Bearing the Autosomal Dopa Decarboxylase Gene in Drosophila melanogaster and Analysis of Ddc Mosaics | Zendy

Donald A. Gailey | Zendy; Deborah L Bordne | Zendy; Ana María Vallés | Zendy; Jeffrey C. Hall | Zendy; K. Andrew White | Zendy

Open Access

Construction of an Unstable Ring-X Chromosome Bearing the Autosomal Dopa Decarboxylase Gene in Drosophila melanogaster and Analysis of Ddc Mosaics

Author(s) -

Donald A. Gailey,

Deborah L Bordne,

Ana María Vallés,

Jeffrey C. Hall,

K. Andrew White

Publication year - 1987

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/115.2.305

Subject(s) - biology , ring chromosome , genetics , ring (chemistry) , drosophila melanogaster , microbiology and biotechnology , x chromosome , chromosome , mutant , gene , karyotype , chemistry , organic chemistry

An unstable Ring-X chromosome, Ddc +- Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed. The construction involved a double recombination event between the unstable Ring-X, R(1)wvC and a Rod-X chromosome which contained a P-element mediated Ddc + insert. The resulting Ddc +-Ring-X chromosome behaves similarly to the parent chromosome with respect to somatic instability. The Ddc +-Ring-X chromosome was used to generate Ddc mosaics. Analyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc + expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.

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