GENETIC REGULATION OF SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE IN BROWN ADIPOSE TISSUE | Zendy

Jeffry R. Cook | Zendy; U Stadler | Zendy; Donna Burkart | Zendy; Leslie P. Kozak | Zendy

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GENETIC REGULATION OF SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE IN BROWN ADIPOSE TISSUE

Author(s) -

Jeffry R. Cook,

U Stadler,

Donna Burkart,

Leslie P. Kozak

Publication year - 1986

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/114.2.567

Subject(s) - biology , dehydrogenase , brown adipose tissue , microbiology and biotechnology , locus (genetics) , northern blot , enzyme , gene , messenger rna , adipose tissue , biochemistry

The levels of sn-glycerol-3-phosphate dehydrogenase (GPDH) were determined in the brown adipose tissue (BAT) of different inbred strains of mice. The BAT of the BALB/cJ strain contains twice as much enzyme activity per milligram protein as do other strains. The appearance of this difference is developmentally dependent, since it is not detected in BAT until 25-30 days postpartum. Genetic analysis of this strain difference has shown that the mechanism of inheritance involves at least two genes, one of which is linked to the Gdc-1 structural locus on chromosome 15. Determinations of GPDH synthesis by immunoprecipitation of GPDH protein labeled in vivo with [3H]leucine, and of GPDH mRNA by Northern blot analysis, establish that in BALB/cJ mice higher rates of enzyme synthesis are determined by elevated levels of GPDH mRNA. It was also found that cold stress increases GPDH mRNA levels in all the strains examined.

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