AGROBACTERIUM-MEDIATED GENE TRANSFER RESULTS MAINLY IN TRANSGENIC PLANTS TRANSMITTING T-DNA AS A SINGLE MENDELIAN FACTOR | Zendy

Françoise Budar | Zendy; Lin Thia-Toong | Zendy; Marc Van Montagu | Zendy; Jean-Pierre Hernálsteens | Zendy

Open Access

AGROBACTERIUM-MEDIATED GENE TRANSFER RESULTS MAINLY IN TRANSGENIC PLANTS TRANSMITTING T-DNA AS A SINGLE MENDELIAN FACTOR

Author(s) -

Françoise Budar,

Lin Thia-Toong,

Marc Van Montagu,

Jean-Pierre Hernálsteens

Publication year - 1986

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/114.1.303

Subject(s) - biology , genetics , selfing , agrobacterium tumefaciens , kanamycin , mendelian inheritance , gene , plasmid , locus (genetics) , transformation (genetics) , genetically modified crops , dna , agrobacterium , transgene , population , demography , sociology

Forty-four independent transformed tobacco plants were obtained from a cocultivation experiment with Agrobacterium tumefaciens strains carrying modified Ti-plasmids. The transformed plants were either self-fertilized or crossed with nontransformed plants or with other transformed plants. The segregation of a phenotypic marker (kanamycin resistance) in the progenies of these plants was determined. In 40 cases out of 44, the segregation of the kanamycin resistance marker is consistent with Mendelian genetics. Among these 40 clones, 35 contain a single kanamycin resistance locus. The five others segregate two independent resistance loci. In two of the single insert clones, the segregation ratio after selfing indicates that the T-DNA insertion may have caused a recessive lethal mutation.

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