HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION | Zendy

Sheldon I. Feinstein | Zendy; K. Brooks Low | Zendy

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HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION

Author(s) -

Sheldon I. Feinstein,

K. Brooks Low

Publication year - 1986

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/113.1.13

Subject(s) - biology , recombination , genetics , allele , escherichia coli , homologous recombination , mutant , mutation , recombinant dna , genetic recombination , microbiology and biotechnology , gene

Using a direct enrichment and screening procedure, mutants of Escherichia coli have been isolated in which recombination frequencies for several intragenic Hfr X F- crosses are significantly higher (twofold to sixfold) than in the parental strains. These hyper-recombination mutations comprised five new mutS- and one new mutL- allele. Together with other known mut- alleles, they were analyzed for effects on intragenic recombination using several types of crosses. Hyper-recombination was found for mutS-, mutL-, mutH (= mutR)- and mutU (= uvrD)-, with the largest effects seen for certain alleles of uvrD; these resulted in over 20-fold excesses in recombinant production for Hfr X F- crosses and F'-chromosome homogenotization. Spontaneous mutator ability was not always correlated with degree of hyper-recombination.

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