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DNA repair-defective alleles of the mei-9, mei-41, mus-104 and mus-101 loci of Drosophila melanogaster were introduced into stocks bearing the UZ and SZ marker sets. Males with the UZ marker set, z  1 (zeste allele) and w  +(TE) (genetically unstable white allele presumably caused by a transposable element), or the SZ marker set, z  1 and w  +R (semistable white allele caused by partial duplication of the w  + locus plus transposon insert), were exposed to EMS at the first instar. After emergence, adult males bearing red spots on lemon-yellow eyes were scored as flies with somatic reversions of w  +(TE) or w  +R. The relative mutabilities (relative values of reversion frequency at an equal EMS dose) of either w  +(TE) or w  +R in a repair-proficient strain and in mei-9, mei-41, mus-104 and mus-101 strains were 1:?1.2:0.3:0.3:0.7, despite the fact that w  +(TE) reverted two to three times as frequently as w  +R under both the repair-proficient and repair-deficient genetic conditions. Similarly, after treatment with MMS, MNNG and ENNG, w  +(TE) was somatically more mutable in the mei-9 strain and less mutable in the mei-41 and mus-104 strains than in the repair-proficient strain. From these results, we propose that mutagenic lesions produced in DNA by treatment with these chemicals are converted to mutant DNA sequences via the error-prone repair mechanisms dependent on the products of the genes mei-41  + (mei-41 and mus-104 being alleles of the same locus) and mus-101  +, whereas they are eliminated by mei-9  +-dependent excision repair. In contrast to the approximately linear responses of induced reversions of w  +(TE) with ENNG in the repair-proficient, mei-9, and mei-41 strains, seemingly there were dosage insensitive ranges for induced reversion with MNNG in the repair-proficient and mei-41 strains, but not for reversion in the mei-9 strain; w  +(TE) in the mus-104 strain was virtually nonmutable with MNNG and ENNG. These results suggest that O  6-methylguanine (O  6MeG) produced in DNA with MNNG, but not O  6-ethylguanine produced with ENNG, is almost completely repaired in a low dose range by constitutive activity of DNA O  6MeG transmethylase. From the distribution of clone sizes of spontaneous revertant spots and other data, we propose that both w  +(TE) and w  +R have a similar tendency to spontaneously revert more frequently at early rather than at late developmental stages, probably reflecting a common property of their inserted transposons.

genetics

DNA REPAIR DEPENDENCE OF SOMATIC MUTAGENESIS OF TRANSPOSON-CAUSED <i>WHITE</i> ALLELES IN <i>DROSOPHILA MELANOGASTER</i> AFTER TREATMENT WITH ALKYLATING AGENTS

DNA REPAIR DEPENDENCE OF SOMATIC MUTAGENESIS OF TRANSPOSON-CAUSED WHITE ALLELES IN DROSOPHILA MELANOGASTER AFTER TREATMENT WITH ALKYLATING AGENTS