GENETIC ANALYSIS OF MUTANTS WITH A REDUCED Ca2+-DEPENDENT K+ CURRENT IN PARAMECIUM TETRAURELIA | Zendy

Robert D. Hinrichsen | Zendy; Ed Amberger | Zendy; Yoshiro Saimi | Zendy; Anthony BurgessCassler | Zendy; Ching Kung | Zendy

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GENETIC ANALYSIS OF MUTANTS WITH A REDUCED Ca2+-DEPENDENT K+ CURRENT IN PARAMECIUM TETRAURELIA

Author(s) -

Robert D. Hinrichsen,

Ed Amberger,

Yoshiro Saimi,

Anthony BurgessCassler,

Ching Kung

Publication year - 1985

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/111.3.433

Subject(s) - mutant , paramecium , microinjection , biology , genetics , cytoplasm , wild type , allele , phenotype , mutation , microbiology and biotechnology , gene

Two mutants of Paramecium tetraurelia with greatly reduced Ca2+-dependent K+ currents have been isolated and genetically analyzed. These mutants, designated pantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+ current is almost completely eliminated in these mutants, whereas the Ca2+ current is normal. The two mutants, pntA and pntB, are recessive and unlinked to each other. pntA is not allelic to several other ion-channel mutants of P. tetraurelia. The microinjection of a high-speed supernatant fraction of wild-type cytoplasm into either pantophobiac mutant caused a temporary restoration to the wild-type phenotype.

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