Open AccessMEIOTIC RECOMBINATION AND SPORULATION IN REPAIR-DEFICIENT STRAINS OF YEAST
Author(s) -
Elizabeth L Dowling,
Daniel H. Maloney,
Seymour Fogel
Publication year - 1985
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/109.2.283
Subject(s) - biology , meiosis , heteroduplex , genetics , mitotic crossover , gene conversion , mutant , homologous recombination , recombination , genetic recombination , saccharomyces cerevisiae , flp frt recombination , chromosomal crossover , mutation , dna repair , ploidy , yeast , gene
A genetic system designed to monitor recombination and sporulation in various repair-deficient yeast strains was constructed. Variously heterozygous at seven or eight sites distributed across the genome, the system facilitated sensitive detection of changes in frequency or pattern of meiotic recombination. Ten rad mutants sensitive primarily to UV-irradiation and without terminal blocks in the sporulation process were studied. Seven were defective in excision repair (rad1, rad2, rad3, rad4, rad10, rad14 and rad16), and three were defective in mutagenic repair (rad5, rad9 and rad18). Individually, each mutant displayed behavior consistent with an orthodox meiosis including a wild-type meiotic recombination profile with respect to gene conversion, PMS and intergenic map distances. Accordingly, we conclude that these mutants are without major effect on meiotic heteroduplex formation or correction. However, certain combinations of excision-defective mutants with rad18 exhibited marked ascosporal inviability. Tetraploids homozygous for rad1 and rad18 produce a large proportion of diploid spores containing a recessive lethal.
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