SPECIFIC DELETION OCCURRING IN THE DIRECTED EVOLUTION OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ESCHERICHIA COLI | Zendy

Raymond D. Miller | Zendy; Daniel E. Dykhuizen | Zendy; Louis Green | Zendy; Daniel L. Hartl | Zendy

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SPECIFIC DELETION OCCURRING IN THE DIRECTED EVOLUTION OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ESCHERICHIA COLI

Author(s) -

Raymond D. Miller,

Daniel E. Dykhuizen,

Louis Green,

Daniel L. Hartl

Publication year - 1984

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/108.4.765

Subject(s) - biology , escherichia coli , genetics , dehydrogenase , gene , mutation , microbiology and biotechnology , enzyme , biochemistry

A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS 5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.

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