ISOGENIC STRAINS OF PHYCOMYCES BLAKESLEEANUS SUITABLE FOR GENETIC ANALYSIS | Zendy

María Isabel Álvarez | Zendy; Arturo P. Eslava | Zendy

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ISOGENIC STRAINS OF PHYCOMYCES BLAKESLEEANUS SUITABLE FOR GENETIC ANALYSIS

Author(s) -

María Isabel Álvarez,

Arturo P. Eslava

Publication year - 1983

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/105.4.873

Subject(s) - phycomyces , phycomyces blakesleeanus , biology , backcrossing , meiosis , genetics , tetrad , mating type , chromosomal crossover , chiasma , genetic recombination , strain (injury) , recombination , gene , botany , mutant , anatomy

The progeny of crosses between wild-type strains of Phycomyces usually do not exhibit all of the expected genotypes from meiosis. By backcrossing, we have isolated a new (+) mating-type strain, A56, which is nearly isogenic with the (-) wild-type NRRL1555 commonly used in Phycomyces research. Tetrad analysis of the backcrosses shows that meiosis becomes more regular as the parental (+) and (-) strains become more isogenic. In our two-factor crosses with unlinked markers, the regularity of meiosis is measured as the percent of reciprocal ditypes plus tetratypes in the progeny. We have shown that this percentage increases from about 15% for crosses between nonisogenic parents to 90% in the eighth backcross. The results indicate that routine, reliable recombination analyses are possible in P. blakesleeanus.

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