TRANSDUCTIONAL INSTABILITY OF Tn5-INDUCED MUTATIONS: GENERALIZED AND SPECIALIZED TRANSDUCTION OF Tn5 BY BACTERIOPHAGE P1 | Zendy

Claire M. Berg | Zendy; Carmen A Grullón | Zendy; Aoquan Wang | Zendy; William Whalen | Zendy; Douglas E. Berg | Zendy

Open Access

TRANSDUCTIONAL INSTABILITY OF Tn5-INDUCED MUTATIONS: GENERALIZED AND SPECIALIZED TRANSDUCTION OF Tn5 BY BACTERIOPHAGE P1

Author(s) -

Claire M. Berg,

Carmen A Grullón,

Aoquan Wang,

William Whalen,

Douglas E. Berg

Publication year - 1983

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/105.2.259

Subject(s) - transduction (biophysics) , biology , transposase , genetics , transposable element , bacteriophage , bacteriophage mu , mutant , mutation , kanamycin , dna transposable elements , gene , escherichia coli , biochemistry

Generalized transduction is commonly used to move transposon-induced mutations among bacterial strains by selecting for inheritance of a transposonencoded resistance determinant. Although complete cotransduction of the resistance determinant and the chromosomal mutation might be expected, it is often found that when Tn5(Kan) insertion mutations are transduced by bacteriophage P1 most of the nonmutant kanamycin-resistant transductants are due to specialized transduction of Tn5. Such P1::Tn5 specialized transducing phage are not found when a mutant Tn5 element lacking a functional transposase is employed.

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