USE OF NYSTATIN-RESISTANT MUTATIONS IN PARASEXUAL GENETIC ANALYSIS IN DICTYOSTELIUM DISCOIDEUM | Zendy

Durgadas P. Kasbekar | Zendy; Sanford J. Madigan | Zendy; Eugene R. Katz | Zendy

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USE OF NYSTATIN-RESISTANT MUTATIONS IN PARASEXUAL GENETIC ANALYSIS IN DICTYOSTELIUM DISCOIDEUM

Author(s) -

Durgadas P. Kasbekar,

Sanford J. Madigan,

Eugene R. Katz

Publication year - 1983

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/104.2.271

Subject(s) - complementation , biology , nystatin , genetics , mutation , dictyostelium discoideum , pleiotropy , gene , genetic analysis , selection (genetic algorithm) , allele , phenotype , artificial intelligence , computer science , antibiotics

Nystatin-resistant mutations exhibit extreme sensitivity to 1.3 mm coumarin. The mutations fall into three complementation groups so it is possible to select for nonallelic mutations conferring sensitivity to coumarin by selection on nystatin-containing nutrient agar plates. Complementation between such coumarin-sensitive mutations allows the selection of diploids on coumarin-containing nutrient agar. Two of the nystatin resistance genes, nysB and nysC, have been mapped tentatively to the previously unmarked linkage group V.

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