FORMATION OF F'TRP PLASMIDS IN ESCHERICHIA COLI K12
Author(s) -
Frédérique Van Gijsegem,
Ariane Toussaint
Publication year - 1982
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/100.3.359
Subject(s) - plasmid , biology , escherichia coli , transposition (logic) , genetics , gene , philosophy , linguistics
From strains carrying two different F-prime factors, we recovered F′ derivatives that acquired the trp chromosomal region. These F′trp plasmids can be isolated at a frequency of 10-5 to 10-6. They were characterized genetically by looking at the size of the trp segment they acquired and at the location of that segment in the parental F′ plasmid. Results are discussed in relationship to possible transposition mechanisms.
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