Open AccessClostridium difficile toxin B intoxicated mouse colonic epithelial CT26 cells stimulate the activation of dendritic cells
Author(s) -
TingYing Huang,
Gregorio Pérez-Cordón,
Lianfa Shi,
Guangchao Li,
Xingmin Sun,
Xiaoning Wang,
Jufang Wang,
Hanping Feng
Publication year - 2015
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1093/femspd/ftv008
Subject(s) - clostridium difficile toxin a , secretion , pseudomembranous colitis , microbiology and biotechnology , clostridium difficile , biology , intestinal mucosa , intestinal epithelium , clostridium difficile toxin b , immunology , epithelium , medicine , antibiotics , biochemistry , genetics
Clostridium difficile causes antibiotic-associated diarrhea and pseudomembranous colitis mainly through two exotoxins TcdA and TcdB that target intestinal epithelial cells. Dendritic cells (DCs) play an important role in regulating intestinal inflammatory responses. In the current study, we explored the interaction of TcdB-intoxicated epithelial cells with mouse bone marrow-derived DCs. TcdB induced cell death and heat shock protein translocation in mouse intestinal epithelial CT26 cells. The intoxicated epithelial cells promoted the phagocytosis and the TNF-α secretion by DCs. Incubation with TcdB-intoxicated CT26 cells stimulated DC maturation. Moreover, TcdB-treated CT26 cells induced DC immigration when they were injected into mice subcutaneously. Taken together, these data demonstrate that TcdB-intoxicated intestinal epithelial cells are able to stimulate DC activation in vitro and attract DCs in vivo, indicating that epithelial cells may be able to regulate DC activation under the exposure of TcdB during C. difficile infection.