Ca2+ stabilization of respiratory complex I from Escherichia coli | Zendy

Galina Belevich | Zendy; Nikolai Belevich | Zendy; Juho Knuuti | Zendy; Marina Verkhovskaya | Zendy

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Ca2+ stabilization of respiratory complex I from Escherichia coli

Author(s) -

Galina Belevich,

Nikolai Belevich,

Juho Knuuti,

Marina Verkhovskaya

Publication year - 2018

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fny097

Subject(s) - chemistry , intracellular , oxidoreductase , escherichia coli , enzyme , biochemistry , biophysics , membrane , dilution , biology , physics , gene , thermodynamics

Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular stabilization factor (ISF). The action of both, Ca2+ and ISF, on Complex I stability is interdependent. The data are discussed in terms of a release of structural Ca2+ as a reason for Complex I decay and an effect of ISF on the affinity and/or accessibility of Ca2+-binding site.

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