Functional characterization of the manganese transporter smf2 homologue gene, PsMnt, of Phanerochaete sordida YK-624 via homologous overexpression | Zendy

Toshio Mori | Zendy; Yuki Nagai | Zendy; Hirokazu Kawagishi | Zendy; Hirofumi Hirai | Zendy

Open Access

Functional characterization of the manganese transporter smf2 homologue gene, PsMnt, of Phanerochaete sordida YK-624 via homologous overexpression

Author(s) -

Toshio Mori,

Yuki Nagai,

Hirokazu Kawagishi,

Hirofumi Hirai

Publication year - 2018

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fny050

Subject(s) - mutant , manganese peroxidase , gene , wild type , peroxidase , phanerochaete , yeast , microbiology and biotechnology , biology , transporter , strain (injury) , manganese , chemistry , biochemistry , enzyme , organic chemistry , anatomy

A homologue gene of the yeast natural resistance-associated macrophage protein (Nramp) family transporter smf2 was identified in the white-rot fungus Phanerochaete sordida YK-624. Relative expression levels of the homologue, designated PsMnt, were roughly equivalent in cultures containing 0 to 1000 μM Mn(II), a concentration non-toxic to the fungus. In the PsMnt-overexpressing mutant, cellular Mn accumulation and manganese peroxidase (MnP) activity increased significantly in 4-day cultures containing 10 μM MnSO4. Compared with the wild-type strain, MnP activity in the overexpressing mutants was higher at lower Mn concentrations (specifically 10-15 times higher). These results suggest that PsMnt is a high-affinity Mn transporter involved in cellular Mn accumulation under Mn-deficient conditions. This is the first report of an smf2 homologue in wood rot fungi.

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