Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters | Zendy

Jana Zemančíková | Zendy; Michala Dušková | Zendy; Hana Elicharová | Zendy; Klára Papoušková | Zendy; Hana Sychrová | Zendy

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Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters

Author(s) -

Jana Zemančíková,

Michala Dušková,

Hana Elicharová,

Klára Papoušková,

Hana Sychrová

Publication year - 2018

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fny020

Subject(s) - yeast , saccharomyces cerevisiae , wine , biology , glycerol , gene , cloning (programming) , mutant , biochemistry , genetics , food science , computer science , programming language

Dekkera bruxellensis is important for lambic beer fermentation but is considered a spoilage yeast in wine fermentation. We compared two D. bruxellensis strains isolated from wine and found that they differ in some basic properties, including osmotolerance. The genomes of both strains contain two highly similar copies of genes encoding putative glycerol-proton symporters from the STL family that are important for yeast osmotolerance. Cloning of the two DbSTL genes and their expression in suitable osmosensitive Saccharomyces cerevisiae mutants revealed that both identified genes encode functional glycerol uptake systems, but only DbStl2 has the capacity to improve the osmotolerance of S. cerevisiae cells.

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