Open AccessThe transcriptional factor TtsI is involved in a negative regulation of swimming motility inMesorhizobium lotiMAFF303099
Author(s) -
Cecilia M. Duarte,
Laura A. Basile,
Andrés Zalguizuri,
Viviana C. Lepek
Publication year - 2016
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1093/femsle/fnw222
Subject(s) - biology , motility , flagellum , rhizobia , microbiology and biotechnology , type three secretion system , regulator , gene , regulation of gene expression , secretion , gene expression , genetics , mutant , bacteria , biochemistry , symbiosis
Mesorhizobium loti MAFF303099 has a functional Type III secretion system (T3SS) that is involved in the determination of competitiveness for legume nodulation. Here we demonstrate that the transcriptional factor TtsI, which positively regulates T3SS genes expression, is involved in a negative regulation of M. loti swimming motility in soft-agar. Conditions that induce T3SS expression affect flagella production. The same conditions also affect promoter activity of M. loti visN gene, a homolog to the positive regulator of flagellar genes that has been described in other rhizobia. Defects in T3SS complex assembly at membranes limited the negative regulation of motility by the expression of TtsI.
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