Impact ofpnpR, a LysR-type regulator-encoding gene, on the cellular processes ofPseudomonas putidaDLL-E4 | Zendy

Qiongzhen Chen | Zendy; Hui Tu | Zendy; Fei Huang | Zendy; Yicheng Wang | Zendy; Weiliang Dong | Zendy; Wenhui Wang | Zendy; Zhoukun Li | Zendy; Fei Wang | Zendy; Zhongli Cui | Zendy

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Impact ofpnpR, a LysR-type regulator-encoding gene, on the cellular processes ofPseudomonas putidaDLL-E4

Author(s) -

Qiongzhen Chen,

Hui Tu,

Fei Huang,

Yicheng Wang,

Weiliang Dong,

Wenhui Wang,

Zhoukun Li,

Fei Wang,

Zhongli Cui

Publication year - 2016

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fnw110

Subject(s) - pseudomonas putida , biology , operon , catabolite repression , biochemistry , gene , transcriptional regulation , serine , gene expression , microbiology and biotechnology , mutant , enzyme

LysR-type transcriptional regulators (LTTRs) regulate various cellular processes in bacteria. pnpR is an LTTR-encoding gene involved in the regulation of hydroquinone (HQ) degradation, and its effects on the cellular processes of Pseudomonas putida DLL-E4 were investigated at the physiological, biochemical and molecular levels. Reverse transcription polymerase chain reaction revealed that pnpR positively regulated its own expression and that of the pnpC1C2DECX1X2 operon; additionally, pnpR partially regulated the expression of pnpA when P. putida was grown on para-nitrophenol (PNP) or HQ. Strains DLL-E4 and DLL-ΔpnpR exhibited similar cellular morphologies and growth rates. Transcriptome analysis revealed that pnpR regulated the expression of genes in addition to those involved in PNP degradation. A total of 20 genes were upregulated and 19 genes were downregulated by at least 2-fold in strain DLL-ΔpnpR relative to strain DLL-E4. Bioinformatic analysis revealed putative PnpR-binding sites located in the upstream regions of genes involved in PNP degradation, carbon catabolite repression and other cellular processes. The utilization of L-aspartic acid, L-histidine, L-pyroglutamic acid, L-serine, γ-aminobutyric acid, D,L-lactic acid, D-saccharic acid, succinic acid and L-alaninamide was increased at least 1.3-fold in strain DLL-ΔpnpR as shown by BIOLOG assays, indicating that pnpR plays a potential negative regulation role in the utilization of carbon sources.

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