Lactobacillus acidophilusbinds to MUC3 component of cultured intestinal epithelial cells with highest affinity | Zendy

Jugal Kishore Das | Zendy; Rajani Kanta Mahapatra | Zendy; Shubhransu Patro | Zendy; Chandan Goswami | Zendy; Mrutyunjay Suar | Zendy

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Lactobacillus acidophilusbinds to MUC3 component of cultured intestinal epithelial cells with highest affinity

Author(s) -

Jugal Kishore Das,

Rajani Kanta Mahapatra,

Shubhransu Patro,

Chandan Goswami,

Mrutyunjay Suar

Publication year - 2016

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fnw050

Subject(s) - lactobacillus acidophilus , lactobacillus fermentum , mucin , lactobacillus plantarum , mucus , microbiology and biotechnology , lactobacillus , adhesion , in vitro , in silico , chemistry , bacterial adhesin , biology , probiotic , bacteria , biochemistry , escherichia coli , lactic acid , ecology , genetics , organic chemistry , gene

Lactobacillus strains have been shown to adhere to the mucosal components of intestinal epithelial cells. However, established in vitro adhesion assays have several drawbacks in assessing the adhesion of new Lactobacillus strains. The present study aimed to compare the adhesion of four different Lactobacillus strains and select the most adherent microbe, based on in silico approach supported by in vitro results. The mucus-binding proteins in Lactobacillus acidophilus, L. plantarum, L. brevis and L. fermentum were identified and their capacities to interact with intestinal mucin were compared by molecular docking analysis. Lactobacillus acidophilus had the maximal affinity of binding to mucin with predicted free energy of -6.066 kcal mol(-1) Further, in vitro experimental assay of adhesion was performed to validate the in silico results. The adhesion of L. acidophilus to mucous secreting colon epithelial HT-29 MTX cells was highest at 12%, and it formed biofilm with maximum depth (Z = 84 μm). Lactobacillus acidophilus was determined to be the most adherent strain in the study. All the Lactobacillus strains tested in this study, displayed maximum affinity of binding to MUC3 component of mucus as compared to other gastrointestinal mucins. These findings may have importance in the design of probiotics and health care management.

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