Open AccessSimultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production byClostridium saccharobutylicumDSM 13864
Author(s) -
Jinjun Dong,
Ji-Cai Ding,
Yun Zhang,
Li Ma,
Guochao Xu,
Ruizhi Han,
Ye Ni
Publication year - 2016
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1093/femsle/fnw003
Subject(s) - corn stover , fermentation , chemistry , butanol , hydrolysis , clostridium acetobutylicum , corn steep liquor , biomass (ecology) , bioreactor , food science , stover , biofuel , enzymatic hydrolysis , clostridium beijerinckii , pulp and paper industry , microbiology and biotechnology , biochemistry , agronomy , ethanol , biology , organic chemistry , engineering , crop
Simultaneous saccharification and fermentation (SSF) process was applied for biobutanol production by Clostridium saccharobutylicum DSM 13864 from corn stover (CS). The key influential factors in SSF process, including corn steep liquor concentration, dry biomass and enzyme loading, SSF temperature, inoculation size and pre-hydrolysis time were optimized. In 5-L bioreactor with SSF process, butanol titer and productivity of 12.3 g/L and 0.257 g/L/h were achieved at 48 h, which were 20.6% and 21.2% higher than those in separate hydrolysis and fermentation (SHF), respectively. The butanol yield reached 0.175 g/g pretreated CS in SSF, representing 50.9% increase than that in SHF (0.116 g/g pretreated CS). This study proves the feasibility of efficient and economic production of biobutanol from CS by SSF.
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