Proof of the triple prerequisite conditions which are essential for carbapenem resistance development in Klebsiella pneumoniae by using radiation-mediated mutagenesis | Zendy

Naina Adren Pinto | Zendy; Roshan D’Souza | Zendy; Le Phuong Nguyen | Zendy; Thao Nguyen Vu | Zendy; Dongeun Yong | Zendy

Open Access

Proof of the triple prerequisite conditions which are essential for carbapenem resistance development in Klebsiella pneumoniae by using radiation-mediated mutagenesis

Author(s) -

Naina Adren Pinto,

Roshan D’Souza,

Le Phuong Nguyen,

Thao Nguyen Vu,

Dongeun Yong

Publication year - 2020

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1093/femsle/fnaa189

Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , carbapenem , biology , bacteria , mutagenesis , enterobacteriaceae , antibiotic resistance , drug resistance , gene , escherichia coli , antibiotics , genetics , mutant

Evolution of multi-drug resistant bacteria has led to worldwide research to better understand the various resistance mechanisms in these strains. Every year, novel information on carbapenem resistance and its mechanisms is being discovered. In this study, radiation-mediated mutagenesis was used to transform a carbapenem-resistant Klebsiella pneumoniae strain to a carbapenem-susceptible bacterium. Through this process, we proved three conditions of loss of the OmpK35 and the OmpK36 genes and acquisition of blaCMY-10 worked together to produce carbapenem resistance in K. pneumoniae. Loss of only one of the porins did not evoke carbapenem resistance. This is the first report on the essential contribution of these three components of carbapenem resistance in K. pneumoniae.

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