Early root alterations after orthodontic force application studied by light and scanning electron microscopy

The purpose of the study was to characterize root surface alterations in orthodontically moved teeth. Thirty-six 40-50-day-old male Wistar rats were used. The maxillary right first molar was mesialized by means of a fixed appliance, exerting 50 g of force upon insertion. One, 2 and 4 days after force application the animals were sacrificed (nine animals per observation period) and block sections processed for analysis. Nine animals served as untreated controls. In total, 20 specimens were prepared for examination of the mesial aspect of the mesiobuccal root by scanning electron microscopy (SEM). The remaining specimens were processed for light microscopy. Three morphologically distinct types of resorption defect were observed: isolated small lacunae, wide shallow resorption bays, and deep resorption lacunae. The area occupied by each resorption type varied significantly (P < 0.05) with time. Isolated small lacunae were the earliest to be observed, sometimes found in continuity with wide shallow resorption bays. Mononucleated macrophage-like cells were associated with both resorption types. Deeper lacunae extending into the dentine were found at the 4 day observation period. These always occurred within shallow resorption bays and appeared to be created by multinucleated cells. From the sequence of the different root surface defects and associated cell types observed during the development of orthodontically induced root resorption, it may be concluded that different cell types, with different resorptive potential but functionally interrelated, are involved in the successive phases of the process, and that each cell type leaves a characteristic resorption pattern on the root surface.