Bacteria Associated WithPiezodorus guildinii(Hemiptera: Pentatomidae), With Special Reference to Those Transmitted by Feeding | Zendy

Claudia Husseneder | Zendy; JongSeok Park | Zendy; Andréa Howells | Zendy; Chinmay Vijay Tikhe | Zendy; Jeffrey A. Davis | Zendy

Open Access

Bacteria Associated WithPiezodorus guildinii(Hemiptera: Pentatomidae), With Special Reference to Those Transmitted by Feeding

Author(s) -

Claudia Husseneder,

JongSeok Park,

Andréa Howells,

Chinmay Vijay Tikhe,

Jeffrey A. Davis

Publication year - 2016

Publication title -

environmental entomology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.749

H-Index - 89

eISSN - 1938-2936

pISSN - 0046-225X

DOI - 10.1093/ee/nvw112

Subject(s) - biology , pantoea , pantoea agglomerans , hemiptera , erwinia , bacteria , pentatomidae , microbiology and biotechnology , symbiotic bacteria , botany , 16s ribosomal rna , symbiosis , genetics

The redbanded stink bug, Piezodorus guildinii (Westwood) (Hemiptera: Heteroptera: Pentatomidae), is a rapidly growing pest damaging southern US agriculture. Pentatomid stink bugs are known to vector bacterial, fungal, and viral plant diseases. However, bacteria associated with redbanded stink bugs and their vector potential have not yet been assessed. In this study, we 1) cultured and identified bacteria transmitted by feeding of redbanded stink bug and 2) described bacteria from guts of redbanded stink bug individuals using next-generation sequencing of 16S rRNA genes. Nineteen bacteria transmitted by feeding of redbanded stink bug on soybean agar were isolated and identified via Sanger sequencing of near full length 16S RNA genes. The transmitted bacteria belonged to at least a dozen species in eight genera and included potential plant pathogens (Phaseolibacter flectens), plant beneficials (Bacillus atropheus), and possible insect beneficials (Acinetobacter sp. and Citrobacter farmeri). A total of 284,448 reads were captured from Illumina MiSeq sequencing of the uncultured gut bacteria community. Fifty-one putative bacteria species (74% of the estimated total species richness) were identified via matches to NCBI databases. The bacteria metagenome contained potential plant and insect pathogens (Erwinia persicina, E. rhaponici, Brenneria nigrifluens, Ralstonia picketti, and Serratia marcescens) and beneficials (Pantoea dispersa, Klebsiella oxytoca, Clostridium butyricum, and Citrobacter farmeri).

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