Open AccessSequence Analysis of a 25-kb Segment in the 17 -19 Region of the Bacillus subtilis Chromosome Containing ada Locus
Author(s) -
Hongtu Liu,
Koki Haga,
Yasuhiro Kasahara,
Nagahisa Ogasawara,
Hideo Takahashi,
Hideki Yoshikawa
Publication year - 1997
Publication title -
dna research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 98
eISSN - 1756-1663
pISSN - 1340-2838
DOI - 10.1093/dnares/4.5.325
Subject(s) - orfs , biology , bacillus subtilis , operon , genetics , gene , homology (biology) , nucleic acid sequence , locus (genetics) , genome , sequence analysis , open reading frame , consensus sequence , peptide sequence , bacteria , escherichia coli
As a part of the Bacillus subtilis genome sequencing project, we have determined a 25-kb sequence covering the 17 degrees-19 degrees region. This region contains 26 complete open reading frames (ORFs) including the alkA and adaA/B operon, which encode genes for adaptive response to DNA alkylation. A homology search for the newly identified 21 ORFs revealed that 4 of them exhibit a significant similarity to known proteins, e.g., methicillin-resistant Staphylococcus aureus (MRSA) protein homolog, proteins involved in chloramphenicol resistance, glucosamine synthase and an ABC transporter protein. The remaining 17 ORFs did not show any significant sequence similarities to known gene products in the database.