Open AccessCloning, Sequencing and Mapping of a Manganese Superoxide Dismutase Gene of the Nematode Caenorhabditis elegans
Author(s) -
Norio Suzuki,
Kentaro Inokuma,
Kayo Yasuda,
Nobuyoshi Ishii
Publication year - 1996
Publication title -
dna research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 98
eISSN - 1756-1663
pISSN - 1340-2838
DOI - 10.1093/dnares/3.3.171
Subject(s) - biology , gene , caenorhabditis elegans , superoxide dismutase , polytene chromosome , genetics , coding region , intron , microbiology and biotechnology , chromosome , biochemistry , enzyme
We have cloned, sequenced and mapped a gene (sod-2) encoding manganese superoxide dismutase [EC 1.15.1.1] from the nematode Caenorhabditis elegans. The sod-2 was mapped to chromosome I by hybridization with a YAC polytene filter. The protein-coding region spans 1129 base pairs including 4 introns and encodes a protein of 221 amino acids (aa) (M(r) = 24536) of which the first 24 aa are the presumed mitochondorial-targeting signal peptide. The gene sequence of sod-2 was slightly different from an isoform, sod-3.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom