The Effect of thecrp Genotypes on the Transformation Efficiency in Escherichia coli | Zendy

Aiko Umemoto | Zendy

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The Effect of thecrp Genotypes on the Transformation Efficiency in Escherichia coli

Author(s) -

Aiko Umemoto

Publication year - 1996

Publication title -

dna research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.647

H-Index - 98

eISSN - 1756-1663

pISSN - 1340-2838

DOI - 10.1093/dnares/3.2.93

Subject(s) - transformation (genetics) , escherichia coli , transformation efficiency , biology , plasmid , gene , genotype , microbiology and biotechnology , genetics , agrobacterium

We have found that a significant difference exists in transformation efficiency between the crp+/crp- isogenic pair of strains of Escherichia coli, with the efficiency being much higher in crp- than in crp+. The ratio of transformation efficiency between crp+ and crp- strains depends very little on the plasmid size. This observation suggests that the difference of the transformation efficiency is due to mechanisms other than a crp-regulated endonuclease. The crp gene is one of the first specific genes that have been shown to affect transformation efficiency.

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