Genes Encoding the Group I Intron-containing tRNALeu and Subunit L of NADH Dehydrogenase from the Cyanobacterium Synechococcus PCC 6301 | Zendy

Mamoru Sugita | Zendy

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Genes Encoding the Group I Intron-containing tRNALeu and Subunit L of NADH Dehydrogenase from the Cyanobacterium Synechococcus PCC 6301

Author(s) -

Mamoru Sugita

Publication year - 1995

Publication title -

dna research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.647

H-Index - 98

eISSN - 1756-1663

pISSN - 1340-2838

DOI - 10.1093/dnares/2.2.71

Subject(s) - biology , intron , transfer rna , nadh dehydrogenase , gene , synechococcus , open reading frame , exon , genetics , genomic dna , protein subunit , microbiology and biotechnology , nucleic acid sequence , group ii intron , rna , cyanobacteria , peptide sequence , rna splicing , bacteria

A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucleotide sequence of a 2,167-bp portion was determined. The trnL-UAA consists of a 34-bp 5' exon, a 240-bp group I intron and a 50-bp 3' exon. In addition, three open reading frames (ORF1, ORF2 and ORF3) were found in the 5' and 3' flanking regions of trnL-UAA. The predicted protein sequence of ORF3, which is located 74-bp upstream from trnL-UAA on the opposite strand, shows 66.2% amino acid identity to that of the Synechocystis PCC 6803 gene encoding subunit L of NADH dehydrogenase (ndhL).

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